Bis-tris propane (BTP) is a water-soluble buffer substance. Bis-tris propane can be used as a suitable buffer for polymerase chain reaction (PCR). Bis-tris propane can enhance the stability or activity of restriction enzymes[1][2].
[1]. K A Eckert, et al. DNA polymerase fidelity and the polymerase chain reaction. PCR Methods Appl. 1991 Aug;1(1):17-24.
[2]. J R Wenner, et al. Buffer effects on EcoRV kinetics as measured by fluorescent staining and digital imaging of plasmid cleavage. Anal Biochem.