Biotin-tyramide is used for tyramide signal amplification (TSA) which is a powerful, patented technology that significantly enhances both chromogenic and fluorescent signals. TSA is easily integrated into standard nonradioactive in situ hybridization (ISH) or IHC protocols.
TSA is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to catalyze the deposition of a fluorophore-labeled tyramide amplification reagent onto tissue sections or cell preparation surfaces that have been previously blocked with proteins.
Biotin-tyramide use horseradish peroxidase (HRP) to catalyze covalent deposition of biotin labels directly adjacent to the immobilized enzyme. The labeling reaction is quick (less than 10 minutes) and deposited labels can be detected with streptavidin conjugates for imaging in brightfield or fluorescence microscopy.
生物素-酪胺用于酪胺信号放大 (TSA),这是一种强大的专利技术,可显着增强显色和荧光信号。 TSA 很容易集成到标准的非放射性原位杂交 (ISH) 或 IHC 方案中。
TSA 是一种酶介导的检测方法,它使用辣根过氧化物酶 (HRP) 催化荧光团标记的酪胺扩增试剂沉积到先前已用蛋白质封闭的组织切片或细胞制备表面。
生物素-酪胺使用辣根过氧化物酶 (HRP) 催化直接与固定化酶相邻的生物素标记共价沉积。标记反应很快（不到 10 分钟）,沉积的标记可以用链霉亲和素偶联物检测,以便在明场或荧光显微镜下成像。