Biotin-PEG4-azide is non-cleavable, azide-activated biotinylation reagent, that widely applied in protein labeling, detection to molecular imaging and the development of targeted therapeutic agents[1]. With The azide group highly reactive in copper(I)-catalyzed alkyne-azide cycloaddition (CuAAC) reaction, Biotin-PEG4-azide can be used to label proteins with biotin and allow for the detection or capture of proteins using streptavidin[2][3][4].
References:
[1] Wang L, Punzalan C, Bajrami B,et al. Multiplying Identifiability of Clickable Peptides Using One-Pot Tagging of Homologous Biotinyl Azides.Chem Rxiv. 2023 Nov.Version 2.
[2] Zhang X N, Lam A T, Cheng Q Q, et al. Discovery of an NAD+ analogue with enhanced specificity for PARP1. Chem Sci. 2022 Jan 27;13(7):1982-1991.
[3] Chen Y, Wang C. Profiling of Protein Carbonylations in Ferroptosis by Chemical Proteomics. Methods Mol Biol. 2022:2543:141-153.
[4] McKenna S M, Florea B I, Zisterer D M, et al. Probing the metalloproteome: an 8-mercaptoquinoline motif enriches minichromosome maintenance complex components as significant metalloprotein targets in live cells.RSC Chem Biol. 2024 Jun 25;5(8):776-786.
Biotin-PEG4-azide是一种不可切割的叠氮化生物素化试剂，广泛应用于蛋白质标记、分子成像检测和靶向治疗剂的开发等领域[1]。Biotin-PEG4-azide的叠氮化物基团在铜(I)催化的炔-叠氮化物环加成（CuAAC）反应中具有很高的活性，因此Biotin-PEG4-azide可以用生物素标记蛋白质，并允许用链亲和素检测或捕获蛋白质[2][3][4]。