Biotin-4-aminophenol is a compound that fuses biotin with 4-aminophenol. Biotin-4-aminophenol is widely used as a bioconjugate for labeling proteins and nucleic acids, and Biotin-4-aminophenol is a key player in a variety of biochemical assays and molecular biology techniques[1]. Biotin, which is also known as vitamin H or coenzyme R, is a water-soluble B-complex vitamin that’s crucial for cell growth, metabolism, and gene expression[2]. The 4-aminophenol moiety provides a reactive group that can be easily conjugated to other molecules[3]. This makes biotin-4-aminophenol super useful for labeling biomacromolecules (proteins, nucleic acids), allowing for detection and quantification through the powerful streptavidin-biotin interaction.biotin-4-aminophenol is used in all sorts of applications, like enzyme-linked immunosorbent assays (ELISA), fluorescence microscopy, and flow cytometry[1].
References:
[1] Ke M, Yuan X, He A, et al. Spatiotemporal profiling of cytosolic signaling complexes in living cells by selective proximity proteomics. Nat Commun. 2021 Jan 4;12(1):71.
[2] Said HM. Biotin: biochemical, physiological and clinical aspects. Subcell Biochem. 2012;56:1-19.
[3] Murakami K, Yoshino M. Prooxidant activity of aminophenol compounds: copper-dependent generation of reactive oxygen species. Biometals. 2022 Apr;35(2):329-334.
Biotin-4-aminophenol是一种将生物素与 4-氨基苯酚融合的化合物。Biotin-4-aminophenol被广泛用作标记蛋白质和核酸的生物偶联物，Biotin-4-aminophenol是各种生化测定和分子生物学技术的关键参与者[1]。生物素也称为维生素 H 或辅酶 R，是一种水溶性 B 族复合维生素，对细胞生长、新陈代谢和基因表达至关重要[2]。4-氨基苯酚部分提供了一个反应性基团，可以很容易地与其他分子偶联[3]。这使得Biotin-4-aminophenol对标记生物大分子（蛋白质、核酸）非常有用，允许通过强大的链霉亲和素-生物素相互作用进行检测和定量，Biotin-4-aminophenol用于各种应用，如酶联免疫吸附测定 （ELISA）、荧光显微镜和流式细胞术[1]。