Biotin-16-UTP

Biotin-16-UTP is a biotin-labeled UTP used for in vitro transcription to prepare RNA probes^[1]. Biotin-16-UTP is an active substrate for RNA polymerase and can be incorporated by a variety of RNA polymerases (including SP6, T3, and T7 RNA polymerases) to form labeled RNA^{[2, 3]}. Biotin-16-UTP-labeled RNA can be detected by reporter molecules linked to streptavidin, avidin, or anti-biotin antibodies^{[4, 5]}. Biotin-16-UTP can be used for molecular hybridization and RNA-protein interaction studies^[6].

References:
[1]McKenney K M, Connacher R P, Dunshee E B, et al. Chemi-Northern: a versatile chemiluminescent northern blot method for analysis and quantitation of RNA molecules[J]. RNA, 2024, 30(4): 448-462.
[2]Chien H, Alston C I, Dix R D. Suppressor of cytokine signaling 1 (SOCS1) and SOCS3 are stimulated within the eye during experimental murine cytomegalovirus retinitis in mice with retrovirus-induced immunosuppression[J]. Journal of Virology, 2018, 92(18): 10.1128/jvi. 00526-18.
[3]Chen M, Zhang L, Guo Y, et al. A novel lncRNA promotes myogenesis of bovine skeletal muscle satellite cells via PFN1‐RhoA/Rac1[J]. Journal of Cellular and Molecular Medicine, 2021, 25(13): 5988-6005.
[4]Ritchey B, Hai Q, Han J, et al. Genetic variant in 3’untranslated region of the mouse pycard gene regulates inflammasome activity[J]. Elife, 2021, 10: e68203.
[5]Bratthauer G L. The avidin–biotin complex (ABC) method and other avidin–biotin binding methods[J]. Immunocytochemical methods and protocols, 2010: 257-270.
[6]Siprashvili Z, Webster D E, Kretz M, et al. Identification of proteins binding coding and non-coding human RNAs using protein microarrays[J]. BMC genomics, 2012, 13: 1-13.

Biotin-16-UTP是一种生物素标记的UTP，用于体外转录制备RNA探针^[1]。Biotin-16-UTP是RNA聚合酶的活性底物，能够通过多种RNA聚合酶（包括SP6、T3和T7 RNA聚合酶）掺入以形成标记的RNA^{[2, 3]}。Biotin-16-UTP标记的RNA可以通过连接到链霉亲和素、亲和素或抗生物素抗体的报告分子来检测^{[4, 5]}。Biotin-16-UTP能够用于分子杂交及RNA-蛋白相互作用研究^[6]。