Biotin-16- dUTP (Biotin-16-deoxyuridine-5'-triphosphate) trisodium can be used to replace its natural counterpart dTTP by enzymatically incorporating it into DNA/cDNA. Biotin-16- dUTP trisodium can be used to produce biotinylated DNA probes in a variety of assay applications.
Biotin-16- dUTP trisodium can be used for in situ detection of DNA fragments by nick end labelling (TUNEL assay) for the study of apoptosis[1].
Biotin-16-dUTP trisodium can be used for replication foci labelling in permeabilised HeLa cell nuclei and detected by fluorescent streptavidin[2].
References:
[1]. C Charriaut-Marlangue, et al. A cautionary note on the use of the TUNEL stain to determine apoptosis. Neuroreport. 1995 Dec 29;7(1):61-4.
[2]. T Krude, et al. Chromatin assembly factor 1 (CAF-1) colocalizes with replication foci in HeLa cell nuclei. Exp Cell Res. 1995 Oct;220(2):304-11.
















