Biotin-11-dUTP is a fluorescent alternative to dTTP that enzymatically non-radioactively labels DNA during PCR, nick translation, cDNA synthesis, random primer labeling, or primer extension[1, 2]. Biotin-11-dUTP can be incorporated into DNA by reverse transcriptase, Taq DNA polymerase, phi29 DNA polymerase, exonuclease-inactive Klenow fragment, Klenow fragment, and DNA polymerase[3, 4, 5].
References:
[1] Green M R, Sambrook J. Preparation of labeled DNA, RNA, and oligonucleotide probes[J]. Cold Spring Harbor Protocols, 2022, 2022(1): pdb. top100578.
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[3] Steck A L. Oligonucleotide-modified Nuclotides[J]. 2013.
[4] Bharati B K, Swetha R K, Chatterji D. Identification and characterization of starvation induced msdgc-1 promoter involved in the c-di-GMP turnover[J]. Gene, 2013, 528(2): 99-108.
[5] Anderson J P, Angerer B, Loeb L A. Incorporation of reporter-labeled nucleotides by DNA polymerases[J]. Biotechniques, 2005, 38(2): 257-264.
Biotin-11-dUTP是dTTP的荧光替代品，在PCR、切口平移、cDNA合成、随机引物标记或引物延伸期间对DNA进行酶促非放射性标记[1, 2]。Biotin-11-dUTP可以通过逆转录酶、Taq DNA聚合酶、phi29 DNA聚合酶、无外切酶活性的Klenow片段、Klenow片段和 DNA聚合酶整合进DNA[3, 4, 5]。