Azide-A-DSBSO crosslinker is a mass spectrometry (MS)-cleavable, membrane-permeable, homobifunctional, azide-labeled, acid-cleavable cross-linked peptide [1]. Azide-A-DSBSO crosslinker carries a bio-orthogonal azide tag that functions as an enrichment handle permitting selective isolation of cross-linked proteins and peptides through azide-based conjugation chemistry and subsequent affinity purification [2]. Azide-A-DSBSO has been used for studying protein-protein interactions (PPI), especially suitable for the study of PPI in living cells[3].
References:
[1] Kaake R M, Wang X, Burke A, et al. A new in vivo cross-linking mass spectrometry platform to define protein–protein interactions in living cells[J]. Molecular & Cellular Proteomics, 2014, 13(12): 3533-3543.
[2] Matzinger M, Kandioller W, Doppler P, et al. Fast and highly efficient affinity enrichment of Azide-A-DSBSO cross-linked peptides[J]. Journal of Proteome Research, 2020, 19(5): 2071-2079.
[3] Burke A M, Kandur W, Novitsky E J, et al. Synthesis of two new enrichable and MS-cleavable cross-linkers to define protein–protein interactions by mass spectrometry[J]. Organic & biomolecular chemistry, 2015, 13(17): 5030-5037.
Azide-A-DSBSO crosslinker是一种质谱(MS)可裂解、膜渗透性、同源双功能、叠氮标记、酸可裂解的交联肽[1]。Azide-A-DSBSO crosslinker携带一个生物正交叠氮标签，该标签可作为富集柄，通过基于叠氮的共轭化学和随后的亲和纯化，实现交联蛋白质和肽的选择性分离[2]。Azide-A-DSBSO crosslinker已用于研究蛋白质-蛋白质相互作用（PPI），尤其适用于活细胞中PPI的研究[3]。