Argireline (Acetyl hexapeptide-3)是一种合成肽，结构模式来源于蛋白SNAP-25的N末端。
Cas No.:616204-22-9
Sample solution is provided at 25 µL, 10mM.
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Argireline (Acetyl hexapeptide-3) is a synthetic peptide that is patterned from the N-terminal end of the protein SNAP-25 [1]. Argireline inhibits vesicle docking by preventing formation of the ternary SNARE complex and by interfering in catecholamine release [2]. Argireline is used in cosmetic formulations to reduce facial muscle contractions, thus preventing the formation of expressive wrinkles, mainly on the forehead and around eyes[3].
In vitro, Argireline treatment for 48 hours significantly inhibited the viability of HEK-293 cells and IMR-32 cells, with IC50 values of 34.862μM and 68.458μM, respectively[4].
References:
[1] Dana A, Rotsztejn H. The peptide argireline-the importance of local application on the skin, in the light of current knowledge[J]. Letters in Drug Design & Discovery, 2017, 14(10): 1215-1220.
[2] Wang Y, Wang M, Xiao S, et al. The anti-wrinkle efficacy of argireline, a synthetic hexapeptide, in Chinese subjects: a randomized, placebo-controlled study[J]. American journal of clinical dermatology, 2013, 14(2): 147-153.
[3] Blanes‐Mira C, Clemente J, Jodas G, et al. A synthetic hexapeptide (Argireline) with antiwrinkle activity[J]. International journal of cosmetic science, 2002, 24(5): 303-310.
[4] Grosicki M, Latacz G, Szopa A, et al. The study of cellular cytotoxicity of argireline-an anti-aging peptide[J]. Acta Biochimica Polonica, 2014, 61(1).
Argireline (Acetyl hexapeptide-3)是一种合成肽，结构模式来源于蛋白SNAP-25的N末端[1]。Argireline通过阻止三元SNARE 复合物的形成并干扰儿茶酚胺的释放来抑制囊泡锚定[2]。Argireline用于化妆品配方中，以减少面部肌肉收缩，从而预防表情纹的产生，尤其是在前额和眼周区域[3]。
在体外，Argireline处理HEK-293细胞和IMR-32细胞48小时，显著抑制了细胞活力，IC50值分别为 34.862μM和68.458μM[4]。
| Cell experiment [1]: | |
Cell lines | HEK-293 cells |
Preparation Method | HEK-293 cells were grown in DMEM medium with 10% (v/v) fetal bovine serum (FBS), 0.2mM glutamine, 100U/ml penicillin, and 100μg/ml streptomycin at 37°C in 5% CO2/atmosphere. Cells (1.5×105 cells/ml) were seeded in 96-well plates allowed to adhere in a 5% CO2 incubator at 37°C overnight. Cells were treated with different concentrations of Argireline (0.01, 0.1, 1, 10, and 100µM) for 48h. The viability was evaluated. |
Reaction Conditions | 0.01, 0.1, 1, 10, and 100µM; 48h |
Applications | Argireline treatment significantly reduced the viability of HEK-293 cells in a dose-dependent manner. |
References: | |
| Cas No. | 616204-22-9 | SDF | |
| 别名 | 六胜肽; Acetyl hexapeptide-3 | ||
| Canonical SMILES | N-acetyl-Glu-Glu-Met-Gln-Arg-Arg-NH2 | ||
| 分子式 | C34H60N14O12S | 分子量 | 888.99 |
| 溶解度 | Water : 8.8 mg/mL (9.90 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 1.1249 mL | 5.6244 mL | 11.2487 mL |
| 5 mM | 225 μL | 1.1249 mL | 2.2497 mL |
| 10 mM | 112.5 μL | 562.4 μL | 1.1249 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >99.00% Appearance: A solid
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