Anti-inflammatory agent 36 is an anti-inflammatory agent. Anti-inflammatory agent 36 inhibits LPS-induced macrophage activation[1].
Anti-inflammatory agent 36 (化合物 5a28) (2.5-20 μM) 抑制 LPS 诱导的活化 RAW 264.7 小鼠巨噬细胞中 TNF-α 和 IL-6 的释放，IC50 为 3.69 (TNF-α) 和 3.68 µM (IL-6)[1]。
Anti-inflammatory agent 36 (10 μM，0.5 小时) 抑制 RAW 264.7 小鼠巨噬细胞中 LPS 诱导的 P-P38 和 P-ERK[1]。
Anti-inflammatory agent 36 (10 μM, 0.5 h) 抑制 LPS 诱导的 TNF-α、IL-6、IL-1β、ICAM-1 和 VCAM-1 的转录[1]。
Western Blot Analysis[1]
| Cell Line: | RAW 264.7 mouse macrophages |
| Concentration: | 10 μM |
| Incubation Time: | 0.5 h |
| Result: | Markedly inhibited P-P38 and P-ERK, indicating the suppression of MAPK signaling. |
Anti-inflammatory agent 36 (化合物 5a28) (10 mg/kg，腹腔注射) 抑制急性肺损伤小鼠模型的炎症[1]。
| Animal Model: | Acute lung injury mouse model[1] |
| Dosage: | 10 mg/kg |
| Administration: | i.p. |
| Result: | Reduced wet/dry weight ratio of the mice lungs. Reduced biomarkers of lymphocytes and macrophages. Suppresses TNF-α, IL-6, IL-1β, 7 VACM-1 and ICAM-1 level. |
[1]. Qian J, et al. Design and synthesis novel di-carbonyl analogs of curcumin (DACs) act as potent anti-inflammatory agents against LPS-induced acute lung injury (ALI). Eur J Med Chem. 2019 Apr 1;167:414-425.