AMT-NHS is an RNA-protein crosslinker. AMT-NHS is composed of a psoralen derivative and an N-droxysuccinimide ester group which react with RNA bases and primary amines of protein, respectively. AMT-NHS can penetrate into living yeast cells and crosslink Cbf5 to H/ACA snoRNAs with high specificity. AMT-NHS induces different crosslinking patterns and targets both single- and double-stranded regions of RNA. AMT-NHS can be used for capturing diverse RNA-protein interactions in cells[1].
[1]. Han Y, et al. Development of an RNA-protein crosslinker to capture protein interactions with diverse RNA structures in cells. RNA. 2022 Mar;28(3):390-399.