AMPPD (Lumi-Phos Plus) is a 1,2-dioxetane derivative that serves as an ultrasensitive chemiluminescent substrate for alkaline phosphatase and is widely used in biochemical detection [1-2]. Under the catalysis of alkaline phosphatase, AMPPD undergoes dephosphorylation to form an unstable intermediate. This intermediate decomposes, leading to cleavage of the dioxetane structure and emission of light at a wavelength of 470nm, with the luminescence intensity being proportional to the concentration of alkaline phosphatase[3-4].
References:
[1] Yue L, Liu YJ. Mechanism of AMPPD Chemiluminescence in a Different Voice. J Chem Theory Comput. 2013 May 14;9(5):2300-12.
[2] Tu L, Wang Y, Yang Y, et al. Spectroscopic study of the authentic emitter of AMPPD chemiluminescence in alkaline aqueous solution. Phys Chem Chem Phys. 2010 Jul 7;12(25):6789-94.
[3] Cate RL, Ehrenfels CW, Wysk M, et al. Genomic Southern analysis with alkaline-phosphatase-conjugated oligonucleotide probes and the chemiluminescent substrate AMPPD. Genet Anal Tech Appl. 1991 May;8(3):102-6.
[3] Cate RL, Ehrenfels CW, Wysk M, et al. Genomic Southern analysis with alkaline-phosphatase-conjugated oligonucleotide probes and the chemiluminescent substrate AMPPD. Genet Anal Tech Appl. 1991 May;8(3):102-6.
[4] Hai Z, Li J, Wu J,, et al. Alkaline Phosphatase-Triggered Simultaneous Hydrogelation and Chemiluminescence. J Am Chem Soc. 2017 Jan 25;139(3):1041-1044.
AMPPD (Lumi-Phos Plus)是一种1,2-二氧环己烷衍生物，作为超灵敏的碱性磷酸酶化学发光底物，广泛应用于生物化学检测中[1-2]。AMPPD在碱性磷酸酶的催化下会脱去磷酸基团，形成一个不稳定的中间体，该中间体分解时二氧杂环丁烷结构断裂并发射出波长为470nm的光，其发光强度与碱性磷酸酶的浓度成正比[3-4]。