Aminoethyl-SS-propionic acid is a bifunctional crosslinker that can couple with carboxylic acid-activated reagents through amino groups or directly react with sulfhydryl groups (-SH) to achieve directional crosslinking of biomolecules[1]. Aminoethyl-SS-propionic acid is a cleavable ADC linker that can be used to synthesize antibody-drug conjugates (ADCs)[2]. Aminoethyl-SS-propionic acid contains a disulfide bond and a terminal amine. The disulfide bond can be cleaved using dithiothreitol (DTT) reagent, and the terminal amine can react with carboxylic acids, activated NHS esters, and other carbonyl compounds[3]. Aminoethyl-SS-propionic acid can be used in the development of targeted drug delivery and controlled release systems[4].
References:
[1] Wong S S, Jameson D M, Wong S S. Chemistry of protein and nucleic acid cross-linking and conjugation[M]. Boca Raton, FL, USA:: CRC Press, 2012.
[2] Beck A, Goetsch L, Dumontet C, et al. Strategies and challenges for the next generation of antibody–drug conjugates[J]. Nature reviews Drug discovery, 2017, 16(5): 315-337.
[3] Koniev O. Development of new bioselective ligation reactions[D]. Université de Strasbourg, 2014.
[4] Hoffman A S, Lai J J. Three significant highlights of controlled drug delivery over the past 55 years: PEGylation, ADCs, and EPR[J]. Adv Drug Deliv Rev, 2020, 158: 2-3.
Aminoethyl-SS-propionic acid是一种双功能交联剂，能够通过氨基与羧酸活化试剂偶联，或直接与巯基（-SH）反应，实现生物分子的定向交联[1]。Aminoethyl-SS-propionic acid是一种可裂解的ADC linker，能够用于合成抗体-药物缀合物（ADC）[2]。Aminoethyl-SS-propionic acid含有二硫键和末端胺，二硫键可以使用二硫代醇（DTT）试剂裂解，末端胺可以与羧酸、活化的NHS酯和其他羰基化合物反应[3]。Aminoethyl-SS-propionic acid能够用于靶向药物递送及可控释放系统的开发[4]。