AMCA N-succinimidyl ester is an amine-reactive fluorescent probe and ester form of AMCA .1 It contains an N-hydroxysuccinimide ester that reacts with lysine residues to form photostable amide links. Upon activation with UV light, AMCA displays emission maxima of 400-460 nm. AMCA N-succinimidyl ester has commonly been used in multiplex immunophenotyping applications.2,3,4
1.Villalobos-Molina, R., Gallardo-OrtÍz, I.A., LÓpez-Guerrero, J.J., et al.Evidence that NAN-190-induced hypotension involves vascular α1-adrenoceptor antagonism in the ratEur. J. Pharmacol.455(1)59-64(2002) 2.Ferri, G.-L., Gaudio, R.M., Castello, I.F., et al.Quadruple immunofluorescence: A direct visualization methodJ. Histochem. Cytochem.45(2)155-158(1997) 3.Nederlof, P.M., Robinson, D., Abuknesha, R., et al.Three-color fluorescence in situ hybridization for the simultaneous detection of multiple nucleic acid sequencesCytometry10(1)20-27(1989) 4.Gothot, A., Grosdent, J.-C., and Paulus, J.-M.A strategy for multiple immunophenotyping by image cytometry: Model studies using latex microbeads labeled with seven streptavidin-bound fluorochromesCytometry24(3)214-225(1996)