Alloisolithocholic acid-d4 is intended for use as an internal standard for the quantification of alloisolithocholic acid by GC- or LC-MS. Alloisolithocholic acid is a metabolite of the secondary bile acid metabolite 3-oxo lithocholic acid (dehydrolithocholic acid).1 It is formed from 3-oxo lithocholic acid by gut microbiota.1 Alloisolithocholic acid (20 ?M) increases the association of the nuclear hormone receptor nerve growth factor IB (Nr4a1) to the gene encoding forkhead box protein P3 (Foxp3) in isolated mouse CD4+ T cells. It induces the differentiation of isolated mouse T cells into regulatory T cells (Tregs) and the production of mitochondrial reactive oxygen species (ROS) in isolated mouse CD4+ T cells when used at a concentration of 20 ?M.2 Alloisolithocholic acid (6 mg/kg) reduces hepatic bile flow and bile salt, cholesterol, and phospholipid secretion, as well as induces loss of microvilli and dilation of canaliculi in hepatocytes, markers of cholestasis, in rats.3 Fecal levels of alloisolithocholic acid are reduced in patients with Crohn’s disease or ulcerative colitis.1
References:
[1]. Li, W., Hang, S., Fang, Y., et al.A bacterial bile acid metabolite modulates Treg activity through the nuclear hormone receptor NR4A1Cell Host Microbe29(9)1366-137.
[7]. e1369(2021).
[2]. Hang, S., Paik, D., Yao, L., et al.Bile acid metabolites control TH17 and Treg cell differentiationNature576(7785)143-148(2019).
[3]. Vonk, R.J., Tuchweber, B., Massé, D., et al.Intrahepatic cholestasis induced by allo monohydroxy bile acid in ratsGastroenterology80(2)242-249(1981).