AkaLumine hydrochloride is a kind of luciferin analog, emits light in the near-infrared (NIR) wavelength range (λmax=677nm) in reactions with native firefly luciferase [1]. AkaLumine hydrochloride has a low Km value for recombinant firefly luciferase protein (2.06μM) and its bioluminescence achieved considerable penetration depths [1].
References:
[1] Kuchimaru T, Iwano S, Kiyama M, et al. A luciferin analogue generating near-infrared bioluminescence achieves highly sensitive deep-tissue imaging [J]. Nature communications, 2016, 7: 11856.
AkaLumine hydrochloride是一种荧光素类似物，在与天然萤火虫荧光素酶发生反应时会在近红外（NIR）波长范围内发光（λmax=677nm）[1]。AkaLumine hydrochloride对重组萤火虫荧光素酶蛋白的Km值较低（2.06μM），其生物发光具有极强的穿透深度[1]。