Adenosine 5'-methylenediphosphate (hydrate)

Adenosine 5'-metlenediphosphate is an inhibitor of ecto-5’-nucleotidase, also known as CD73, with a K_i value of 37 nM.¹ It inhibits cAMP accumulation induced by adenosine 5'-monophosphate , adenosine 5'-diphosphate , or adenosine 5'-triphosphate but not adenosine in VA-13 human fibroblasts when used at a concentration of 100 µM. Adenosine 5'-metlenediphosphate reduces proliferation of U138MG glioma cells, as well as inhibits the invasion and migration of MHCC97H hepatocellular carcinoma (HCC) cells in a migration assay.^2,3 It increases tumor infiltration of CD3⁺CD8⁺ T cells and reduces tumor growth in a K1735 murine melanoma model when administered at a dose of 400 µg/mouse.⁴