Acridinium NHS ester (AE NHS) is a protein and nucleic acid label at primary amines in biomolecules and produce chemiluminescence in the presence of hydrogen peroxide (H2O2)[1] [2].
Acridinium NHS ester is applied in DNA labeling ex vitro(such as 2.95mmol/L, 30min, 37°C)[3], and protein labeling ex vitro (such as 10μM)[4]. Acridinium NHS ester–labeled antibody is also applied in microRNA detection[5], and viral genome of SARS-CoV-2[6].
References:
[1] Arnold, L J Jr et al. “Assay formats involving acridinium-ester-labeled DNA probes.” *Clinical chemistry* vol. 35,8 (1989): 1588-94.
[2] Natrajan, Anand, David Wen, and David Sharpe. "Synthesis and properties of chemiluminescent acridinium ester labels with fluorous tags." *Organic & Biomolecular Chemistry* 12.23 (2014): 3887-3901.
[3] He, Yi et al. “DNA sensor by using electrochemiluminescence of acridinium ester initiated by tripropylamine.” *Analytical and bioanalytical chemistry* vol. 399,10 (2011): 3451-8. doi:10.1007/s00216-010-4157-y
[4] Lai, Yongquan et al. “Using acridinium ester as the sonochemiluminescent probe for labeling of protein.” *The Analyst* vol. 134,1 (2009): 131-7. doi:10.1039/b813330a
[5] Kappel, Andreas et al. “MicroRNA in vitro diagnostics using immunoassay analyzers.” *Clinical chemistry* vol. 61,4 (2015): 600-7. doi:10.1373/clinchem.2014.232165
[6]Pham, Jimmykim et al. “Performance Characteristics of a High-Throughput Automated Transcription-Mediated Amplification Test for SARS-CoV-2 Detection.” *Journal of clinical microbiology* vol. 58,10 e01669-20. 22 Sep. 2020, doi:10.1128/JCM.01669-20
Acridinium NHS ester (AE NHS)能够用于标记蛋白质和核酸上的伯胺，经过过氧化氢（H2O2）反应产生化学发光[1] [2]。
Acridinium NHS酯用于DNA体外标记（如2.95mmol/L, 30min, 37°C）[3]，蛋白质体外标记（如1*10-5 mol/L）[4]。Acridinium NHS酯标记抗体也用于检测microRNA[5]和SARS-CoV-2病毒基因组[6]。