Acridine Orange 10-Nonyl Bromide (Nonylacridine orange) is a mitochondria-targeting fluorescent probe (excitation wavelength: 489nm; emission wavelength: 525nm). When Acridine Orange 10-Nonyl Bromide interacts with cardiolipin, the excitation and emission wavelengths of the dye shift to 450nm and 640nm, respectively. Acridine Orange 10-Nonyl Bromide can be used in related studies such as mitochondrial morphology and localization, quantitative detection of cardiolipin, monitoring of cell apoptosis, and analysis of multidrug resistance[1-4].
References:
[1] Ferlini C, Scambia G. Assay for apoptosis using the mitochondrial probes, Rhodamine123 and 10-N-nonyl acridine orange. Nat Protoc. 2007;2(12):3111-4.
[2] Gohil VM, Gvozdenovic-Jeremic J, Schlame M, et al. Binding of 10-N-nonyl acridine orange to cardiolipin-deficient yeast cells: implications for assay of cardiolipin. Anal Biochem. 2005 Aug 15;343(2):350-2.
[3] Zhang GX, Kimura S, Murao K, et al. Inhibition of cytochrome c release by 10-N-nonyl acridine orange, a cardiolipin-specific dye, during myocardial ischemia-reperfusion in the rat. Am J Physiol Heart Circ Physiol. 2010 Feb;298(2):H433-9.
[4] Garcia Fernandez MI, Ceccarelli D, Muscatello U. Use of the fluorescent dye 10-N-nonyl acridine orange in quantitative and location assays of cardiolipin: a study on different experimental models. Anal Biochem. 2004 May 15;328(2):174-80.
Acridine Orange 10-Nonyl Bromide (Nonylacridine orange)是一种具有线粒体靶向性的荧光探针（激发波长：489nm；发射波长：525nm）。当Acridine Orange 10-Nonyl Bromide与心磷脂相互作用时，染料的激发和发射波长变为450nm和640nm。Acridine Orange 10-Nonyl Bromide可用于线粒体形态学与定位研究、心磷脂定量检测、细胞凋亡监测以及多药耐药性分析等相关研究[1-4]。