GlpBio

A-802715

目录号: GC33419纯度: >98.00%
A802715是一种甲基黄嘌呤衍生物，TD50为0.9-1.1mM。
A-802715
Cas No.: 107767-58-8
规格价格库存数量操作
1mg¥1,440.00现货
1
5mg¥2,880.00现货
1
10mg¥4,590.00现货
1
10mM (in 1mL DMSO)¥2,043.00现货
1
电话: 400-920-5774Email: sales@glpbio.cn

文献被引

本产品暂无引用记录;以下为 GlpBio 产品在 Nature / Cell / Science 等顶刊的客户引用样例
  • Nature cover
    Nature
    641, 529–536 (2025)
  • Nature cover
    Nature
    628, 630–638 (2024)
  • Nature cover
    Nature
    632, 686–694 (2024)
  • Nature cover
    Nature
    618, 1017–1023 (2023)
  • Nature cover
    Nature
    610, 366–372 (2022)
  • Cell cover
    Cell
    187(9):2288-2304 (2024)
  • Cell cover
    Cell
    183(7):1867-1883 (2020)
  • Science cover
    Science
    388(6745) (2025)
  • Science cover
    Science
    387(6739) (2025)
  • Science cover
    Science
    387(6734) (2025)
  • Cell Research cover
    Cell Research
    35, 97–116 (2025)
  • Cell Research cover
    Cell Research
    34, 683–706 (2024)
  • Cell Research cover
    Cell Research
    33, 273–287 (2023)
  • Cell Research cover
    Cell Research
    33, 546–561 (2023)
  • Cell Research cover
    Cell Research
    33, 904–922 (2023)
  • Cell Research cover
    Cell Research
    31, 1291–1307 (2021)

产品描述 Description

A802715 is a methylxanthine derivative. A802715 has a TD50 (toxic dose of 50%) of 0.9-1.1 mM.

The toxicity of the methylxanthine derivative A802715 is determined against the two human melanoma lines, Be11 and MeWo, and against the two human squamous cell carcinoma lines, 4197 and 4451, by vital dye staining assay. A802715 has a TD50 of 0.9-1.1 mM and is the most toxic. In p53 wt cells BrdU incorporations show that the irradiation-induced suppression of S-phase entry is strongly enhanced by A802715. A802715 prolongs the G2/M block or remain ineffective depending on the p53 status of the cell line[1].

[1]. Bohm L, et al. Influence of pentoxifylline, A-802710, propentofylline and A-802715 (Hoechst) on the expression of cell cycle blocks and S-phase content after irradiation damage. Biochim Biophys Acta. 2000 Dec 11;1499(1-2):1-10.

实验参考方法 Experimental Reference Method

Cell experiment:

DNA analysis is performed using a FACScan flow cytometer emitting a 488 nm beam. Red fluorescence from PI emission is collected as a linear signal through a 600 nm bandpass filter and recorded as a measure of total DNA content. Processing the red fluorescence into height, area and width (doublet discrimination mode) eliminated cell doublets. Data are collected in list mode and 10000 events are recorded per sample and displayed as a frequency distribution histogram. Estimates of the percentages of cells in the different periods of postirradiation incubation with marker statistics reveal the time at which the G2/M block is maximally expressed. These times are used for each cell line as a starting point at which the methylxanthine drugs were added. Cell debris, nuclei doublets and triplets were excluded by gating[1].

References:

[1]. Bohm L, et al. Influence of pentoxifylline, A-802710, propentofylline and A-802715 (Hoechst) on the expression of cell cycle blocks and S-phase content after irradiation damage. Biochim Biophys Acta. 2000 Dec 11;1499(1-2):1-10.

产品文档 Product Documents

Purity:>98.00%

化学性质Chemical Properties

CAS 号
107767-58-8
SMILES
O=C(N1CCCCC(C)(O)C)N(C)C2=C(N(CCC)C=N2)C1=O
分子式
C16H26N4O3
分子量
322.4 g/mol
溶解性
DMSO: 190 mg/mL (589.33 mM)
保存条件
Store at -20°C
General tips
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
为了提高溶解度，请将管子加热至 37°C，然后在超声波浴中震荡一段时间。
Shipping Condition
评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备 RT，或根据请求配备蓝冰。

计算工具摩尔浓度 / 稀释 / 分子量 / 单位换算 / 体内配方 / 溶解度

g/mol