7α-hydroxy Cholesterol是一种内源性的氧化胆固醇代谢物。
Cas No.:566-26-7
Sample solution is provided at 25 µL, 10mM.
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7α-hydroxy Cholesterol is an endogenous oxidized cholesterol metabolite. 7α-hydroxy Cholesterol serves as a key rate-limiting intermediate in the bile acid biosynthesis pathway and a potential biomarker for lipid peroxidation. 7α-hydroxy Cholesterol can be used in research related to cardiovascular metabolic diseases such as atherosclerosis, as well as long-acting anti-tumor immunotherapy using TCR-T cells[1-4].
In vitro, 7α-hydroxy Cholesterol (5μg/ml) was applied to treat THP-1 monocytes for 6-48 hours. 7α-hydroxy Cholesterol significantly upregulated the expression of pattern recognition receptors (TLR6 and CD14) and chemokines (CCL2, CCL3, and CCL4). 7α-hydroxy Cholesterol enhanced cellular immune responses to PAMPs (such as FSL-1 and LPS) and immune cell migration, while simultaneously activating the phosphorylation of the Akt, Src, ERK1/2, and NF-κB signaling pathways[5]. 7α-hydroxy Cholesterol (12.5-100ng/ml) was used to treat HeLa cells for 24 hours, followed by stimulation with pyolysin for 2 hours. 7α-hydroxy Cholesterol significantly inhibited lactate dehydrogenase (LDH) leakage and cytolysis. 7α-hydroxy Cholesterol prevented the activation of MAPK cell stress responses and limited alterations in the cytoskeleton[6].
References:
[1] Cho HR, Son Y, Kim SM, et al. 7α-Hydroxycholesterol induces monocyte/macrophage cell expression of interleukin-8 via C5a receptor. PLoS One. 2017 Mar 21;12(3):e0173749.
[2] Crosignani A, Zuin M, Allocca M, et al. Oxysterols in bile acid metabolism. Clin Chim Acta. 2011 Nov 20;412(23-24):2037-45.
[3] Kim BY, Son Y, Kim BJ, et al. Atheroma-Relevant 7-Oxysterols Differentially Upregulate Cd14 Expression. Int J Mol Sci. 2023 Jun 23;24(13):10542.
[4] Nury T, Samadi M, Zarrouk A, et al. Improved synthesis and in vitro evaluation of the cytotoxic profile of oxysterols oxidized at C4 (4α- and 4β-hydroxycholesterol) and C7 (7-ketocholesterol, 7α- and 7β-hydroxycholesterol) on cells of the central nervous system. Eur J Med Chem. 2013;70:558-67.
[5] Son Y, Kim BY, Kim M, et al. Glucocorticoids Impair the 7α-Hydroxycholesterol-Enhanced Innate Immune Response. Immune Netw. 2023 Oct 19;23(5):e40.
[6] Ormsby TJR, Owens SE, Clement L, et al. Oxysterols Protect Epithelial Cells Against Pore-Forming Toxins. Front Immunol. 2022 Jan 26;13:815775.
7α-hydroxy Cholesterol是一种内源性的氧化胆固醇代谢物。7α-hydroxy Cholesterol是胆汁酸生物合成途径中的关键限速中间体及脂质过氧化的潜在生物标志物。7α-hydroxy Cholesterol可用于动脉粥样硬化等心血管代谢疾病以及TCR-T细胞长效抗肿瘤免疫治疗的相关研究[1-4]。
在体外，7α-hydroxy Cholesterol（5μg/ml）处理THP-1单核细胞6-48小时。7α-hydroxy Cholesterol显著上调了模式识别受体（TLR6和CD14）及趋化因子（CCL2、CCL3和CCL4）的表达。7α-hydroxy Cholesterol增强了细胞对PAMPs（如FSL-1和LPS）的免疫应答及免疫细胞迁移，同时激活了Akt、Src、ERK1/2和NF-κB信号通路的磷酸化[5]。7α-hydroxy Cholesterol（12.5-100ng/ml）处理HeLa细胞24小时，随后以pyolysin刺激2h。7α-hydroxy Cholesterol显著抑制乳酸脱氢酶（LDH）的泄漏及细胞溶解，防止MAPK细胞应激反应的激活并限制细胞骨架的改变[6]。
| Cell experiment [1]: | |
Cell lines | HeLa cells (human epithelial immortal cell line) |
Preparation Method | HeLa cells were maintained in complete DMEM supplemented with 10% fetal bovine serum (FBS) at 37.5°C in humidified air with 5% CO₂. HeLa cells were seeded at 4 x 10⁴ cells/well in 24-well plates and cultured until 70% confluent. Then, cells were treated with 7α-hydroxy Cholesterol (12.5-100ng/ml) in serum-free medium. |
Reaction Conditions | 12.5-100ng/ml; 24h |
Applications | 7α-hydroxy Cholesterol reduced pyolysin-induced lactate dehydrogenase (LDH) leakage and cytolysis. 7α-hydroxy Cholesterol also prevented potassium ion leakage, mitogen-activated protein kinase (MAPK) cell stress responses, and limited alterations in the cytoskeleton. |
References: | |
| Cas No. | 566-26-7 | SDF | |
| 别名 | 胆甾-5-烯-3,7二醇 | ||
| 化学名 | cholest-5-ene-3β,7α-diol | ||
| Canonical SMILES | O[C@H](C1)CC[C@@]2(C)C1=C[C@@H](O)[C@]3([H])[C@]2([H])CC[C@@]4(C)[C@@]3([H])CC[C@]4([H])[C@H](C)CCCC(C)C | ||
| 分子式 | C27H46O2 | 分子量 | 402.7 |
| 溶解度 | DMF: 2 mg/ml, DMSO: 0.1 mg/ml, Ethanol: 20 mg/ml | 储存条件 | Store at -20°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.4832 mL | 12.4162 mL | 24.8324 mL |
| 5 mM | 496.6 μL | 2.4832 mL | 4.9665 mL |
| 10 mM | 248.3 μL | 1.2416 mL | 2.4832 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
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| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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- Purity: >98.00% Appearance: A solid
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