7,4′-Di-O-methylapigenin is a flavonoid that has been found in T. diffusa and has diverse biological activities.1,2,3,4,5 It inhibits monoamine oxidase B (MAO-B) but not MAO-A (IC50s = 0.198 and >100 ?M, respectively), as well as p38α MAPK but not JNK3 (IC50s = 27.8 and >100 ?M, respectively).1,2 7,4′-Di-O-methylapigenin inhibits the production of nitric oxide (NO) in LPS-stimulated RAW 264.7 macrophages (IC50 = 24.5 ?M).3 It reduces the growth of C. albicans when used at a concentration of 45 ?g/ml.4 7,4′-Di-O-methylapigenin inhibits carrageenan-induced edema in rats (ED25 = 75 mg/kg).5
1.Chaurasiya, N.D., Zhao, J., Pandey, P., et al.Selective inhibition of human monoamine oxidase B by acacetin 7-methyl ether isolated from Turnera diffusa (damiana)Molecules24(4)810(2019) 2.Goettert, M., Schattel, V., Koch, P., et al.Biological evaluation and structural determinants of p38α mitogen-activated-protein kinase and c-Jun-N-terminal kinase 3 inhibition by flavonoidsChembiochem11(18)(2010) 3.Tewtrakul, S., and Subhadhirasakul, S.Effects of compounds from Kaempferia parviflora on nitric oxide, prostaglandin E2 and tumor necrosis factor-alpha productions in RAW264.7 macrophage cellsJ. Ethnopharmacol.120(1)81-84(2008) 4.Mangoyi, R., Midiwo, J., and Mukanganyama, S.Isolation and characterization of an antifungal compound 5-hydroxy-7,4’-dimethoxyflavone from Combretum zeyheriBMC Complement. Altern. Med.15405(2015) 5.Fourie, R.G., and Snyckers, F.O.A flavone with antiinflammatory activity from the roots of Rhus undulataJ. Nat. Prod.47(6)1057-1058(1984)