5R(6S)-EET is an oxylipin. It depolarizes transepithelial voltage in isolated rabbit renal cortical collecting duct when applied to the luminal side at a concentration of 1 µM to a lesser extent than 5S(6R)-EET .[1] In solution, 5(6)-EET degrades into 5,6-DiHET and 5(6)-δ-lactone, which can be converted to 5(6)-DiHET and quantified by GC-MS.[2]
References:
[1].Sakairi, Y., Jacobson, H.R., Noland, T.D., et al.5,6-EET inhibits ion transport in collecting duct by stimulating endogenous prostaglandin synthesisAm. J. Physiol.268(5 Pt 2)F931-F939(1995).
[2].Rashid, M., Manivet, P., Nishio, H., et al.Identification of the binding sites and selectivity of sarpogrelate, a novel 5-HT2 antagonist, to human 5-HT2A, 5-HT2B and 5-HT2C receptor subtypes by molecular modelingLife Sci.73(2)193-207(2003).