5-phenyl-indole-3-acetic acid (5-Ph-IAA) is a derivative of IAA. 5-Ph-IAA is a ligand to establish the auxin-induced degron 2 system (AID2) together with the OsTIR1 (F74G) mutant. Using AID2, degron fusion proteins can now be more precisely controlled, enabling target protein degradation with a half-life of 10 to 45 minutes by adding low doses of 5-Ph-IAA [1]. 5-Ph-IAA shows higher affinity to AtTIR1(F79G).
The mAID-fused targets were rapidly degraded by the addition of 1 μM 5-Ph-IAA and showed significant tumour suppression of mAID-BRD4 xenografts at all doses of 5-Ph-IAA treatment including 0, 1, 3, 10 mg/kg [1]. The treatment with 50 mM 5-Ph-IAA caused a rapid decrease in fluorescence intensity in many embryos (78%, 18/23 embryos), but 5 mM 5-Ph-IAA did not. 5-Ph-IAA can be used for loss-of-function experiments in C. elegans embryos, although treatment with 5-Ph-IAA is not always effective, possibly because the C. elegans embryo develops within the eggshell, which blocks its permeability to many compounds [2].
References:
[1].Yesbolatova A, Saito Y, Kitamoto N, Makino-Itou H, Ajima R, Nakano R, Nakaoka H, Fukui K, Gamo K, Tominari Y, Takeuchi H, Saga Y, Hayashi KI, Kanemaki MT. The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat Commun. 2020 Nov 11;11(1):5701.
[2].Negishi T, Kitagawa S, Horii N, Tanaka Y, Haruta N, Sugimoto A, Sawa H, Hayashi KI, Harata M, Kanemaki MT. The auxin-inducible degron 2 (AID2) system enables controlled protein knockdown during embryogenesis and development in Caenorhabditis elegans. Genetics. 2022 Feb 4;220(2):iyab218.
5-phenyl-indole-3-acetic acid (5-Ph-IAA) 是 IAA 的衍生物。 5-Ph-IAA 是与 OsTIR1 (F74G) 突变体一起建立生长素诱导的 degron 2 系统 (AID2) 的配体。使用 AID2,现在可以更精确地控制 degron 融合蛋白,通过添加低剂量的 5-Ph-IAA [1] 使目标蛋白降解的半衰期为 10 至 45 分钟。 5-Ph-IAA对AtTIR1(F79G)具有更高的亲和力。
通过添加 1 μM 5-Ph-IAA,mAID 融合靶标会迅速降解,并且在所有剂量的 5-Ph-IAA 处理（包括 0、1、3、10毫克/千克 [1]。用 50 mM 5-Ph-IAA 处理导致许多胚胎（78%,18/23 胚胎）的荧光强度快速降低,但 5 mM 5-Ph-IAA 没有。 5-Ph-IAA 可用于秀丽隐杆线虫胚胎的功能丧失实验,尽管用 5-Ph-IAA 处理并不总是有效,这可能是因为秀丽隐杆线虫胚胎在蛋壳内发育,阻碍了其渗透性许多化合物 [2].