5-methyl-UTP is a modified nucleoside triphosphate that can significantly affect the structure and function of RNA and plays an important role in biological systems[1]. During the in vitro mRNA synthesis process, 5-methyl-UTP can be incorporated into RNA molecules containing methylated uracil[2-3]. 5-methyl-UTP-mediated Methylation modification can enhance the stability and translation efficiency of RNA, reduce RNA degradation, thereby increasing the persistence and effectiveness of gene expression, and shows broad application prospects in the development of new RNA therapies and treatment methods[4]. The application of 5-methyl-UTP is crucial for studying the role of RNA methylation in gene regulation as well as the chemical properties and biological functions of RNA, 5-methyl-UT can be used in the screening of specific hydrolases and research related to nucleotide metabolism[5].
References:
[1] Gao S, Sun Y, Chen X, et al. Pyrimidine catabolism is required to prevent the accumulation of 5-methyluridine in RNA[J]. Nucleic Acids Research, 2023, 51(14): 7451-7464.
[2] NAKAYAMA C, SANEYOSHI M. Utilizations of various uridine 5′-triphosphate analogues by DNA-dependent RNA polymerases I and II purified from liver nuclei of the cherry salmon (Oncorhynchus masou)[J]. The Journal of Biochemistry, 1984, 96(5): 1501-1509.
[3] Nakayama C, Saneyoshi M. Utilization of 5-alkyl UTPs by DNA-dependent RNA polymerase I and II purified from cherry salmon (Onchorhynchus masou) liver[C]//Nucleic Acids Symposium Series. 1980 (8): s193-6.
[4] Meneghesso S, Vanderlinden E, Stevaert A, et al. Synthesis and biological evaluation of pyrimidine nucleoside monophosphate prodrugs targeted against influenza virus[J]. Antiviral research, 2012, 94(1): 35-43.
[5]Xu W L, Shen J Y, Dunn C A, et al. A new subfamily of the Nudix hydrolase superfamily active on 5-methyl-UTP (ribo-TTP) and UTP[J]. Journal of Biological Chemistry, 2003, 278(39): 37492-37496.
5-methyl-UTP是一种能显著影响RNA结构和功能的修饰核苷三磷酸，在生物系统中发挥重要作用[1]。在体外mRNA合成过程中,5-methyl-UTP可被掺入含有甲基化尿嘧啶的RNA分子中[2-3]。5-methyl-UTP介导的甲基化修饰可以增强RNA的稳定性和翻译效率，减少RNA降解，从而增加基因表达的持久性和有效性，在开发新的RNA疗法和治疗方法方面显示出广阔的应用前景[4]。5-methyl-UTP的应用对于研究RNA甲基化在基因调控中的作用以及RNA的化学性质和生物学功能具有重要意义，5-methyl-UTP可用于特异性水解酶的筛选和与核苷酸代谢相关的研究[5]。