5-hme-CTP is a modified cytidine triphosphate with a hydroxymethyl group at the 5-carbon position. It can be used to replace unmodified mRNA and enhance the stability and functionality of RNA[1]. The mRNA modification function of 5-hme-CTP has a wide range of applications in gene expression research, RNA drug development and gene therapy[2]. 5-hme-CTP can reduce the innate immune response of human and other mammalian cells[3, 4].
References:
[1] Fu L, Guerrero C R, Zhong N, et al. Tet-mediated formation of 5-hydroxymethylcytosine in RNA[J]. Journal of the American Chemical Society, 2014, 136(33): 11582-11585.
[2] Kuszczynska A, Bors M, Podskoczyj K, et al. Chemistry of installing epitranscriptomic 5-modified cytidines in RNA oligomers[J]. Organic & Biomolecular Chemistry, 2024, 22(36): 7271-7286.
[3] Lu M, Xue M, Wang H T, et al. Nonsegmented negative-sense RNA viruses utilize N 6-methyladenosine (m6A) as a common strategy to evade host innate immunity[J]. Journal of virology, 2021, 95(9): 10.1128/jvi. 01939-20.
[4] Cui L, Ma R, Cai J, et al. RNA modifications: importance in immune cell biology and related diseases[J]. Signal transduction and targeted therapy, 2022, 7(1): 334.
5-hme-CTP是一种修饰的胞苷三磷酸，其5位碳上带有一个羟甲基，可用于替代未修饰的mRNA，增强RNA的稳定性和功能性[1]。5-hme-CTP对mRNA的修饰功能在基因表达研究、RNA药物开发以及基因治疗中具有广泛应用[2]。5-hme-CTP能够降低人类和其他哺乳动物细胞的先天免疫反应[3, 4]。