5-Formyl-CTP is a modified nucleoside triphosphate that is commonly used to replace naturally occurring cytidine triphosphate (CTP) during in vitro mRNA synthesis and is of great significance in studying RNA metabolism and signaling pathways[1]. 5-Formyl-CTP actively participates in RNA formation as a substrate during transcription, promoting RNA processing, modification, and various cellular processes (such as gene expression and regulation)[2]. 5-Formyl-CTP can act as a nucleoside to participate in regulating the conversion of methylated DNA to unmethylated DNA through oxidative pathways[3, 4].
References:
[1] Steinle H, Ionescu T M, Schenk S, et al. Incorporation of synthetic mRNA in injectable chitosan-alginate hybrid hydrogels for local and sustained expression of exogenous proteins in cells[J]. International Journal of Molecular Sciences, 2018, 19(5): 1313.
[2] Samanta B, Horning D P, Joyce G F. 3′-End labeling of nucleic acids by a polymerase ribozyme[J]. Nucleic acids research, 2018, 46(17): e103-e103.
[3] Poliskey J A. Metabolic stability and persistence of expression of mRNA for nonviral gene delivery[D]. The University of Iowa, 2018.
[4] Iwan K, Rahimoff R, Kirchner A, et al. 5-Formylcytosine to cytosine conversion by C–C bond cleavage in vivo[J]. Nature chemical biology, 2018, 14(1): 72-78.
5-Formyl-CTP（5-甲酰基-CTP）是一种修饰的三磷酸核苷，通常用于在体外mRNA合成过程中替代天然存在的三磷酸胞苷（CTP），在研究RNA代谢和信号通路方面具有重要意义[1]。5-Formyl-CTP在转录过程中作为底物积极参与RNA形成，促进RNA加工、修饰和多种细胞过程（如基因表达和调控）[2]。5-Formyl-CTP能够作为核苷参与通过氧化途径调节甲基化DNA转化为非甲基化DNA[3, 4]。