5-Carboxytetramethylrhodamine is a fluorescent dye that has commonly been used for the covalent labeling of oligonucleotides for DNA analysis.1 It displays excitation and emission maxima of 546 and 580 nm, respectively.2 5-Carboxytetramethylrhodamine has also been used in various fluorescence polarization assays and protein FRET experiments.2,3
1.Kvach, M.V., Stepanova, I.A., Prokhorenko, I.A., et al.Practical synthesis of isomerically pure 5- and 6-carboxytetramethylrhodamines, useful dyes for DNA probesBioconjug. Chem.20(8)1673-1682(2009) 2.Qi, J., Oppenheimer, M., and Sobrado, P.Fluorescence polarization binding assay for Aspergillus fumigatus virulence factor UDP-galactopyranose mutaseEnzyme Res.513905(2011) 3.Casiraghi, A., Longhena, F., Straniero, V., et al.Design and synthesis of fluorescent-methylphenidate analogues for FRET-based assay of synapsin III bindingChemMedChem15(14)1330-1337(2020)