5-(Biotinamido)pentylamine is a biotin derivative with a C5 carbon chain spacer [1]. 5‑(Biotinamido)pentylamine is mainly used as an amine donor substrate for transglutaminase (TG). After being catalyzed by the enzyme, it can covalently bind to the glutamine residues in the protein through a γ‑amide bond, thereby introducing the biotin tag into the target protein [2-3].
References:
[1]. Ikura K, Kita K, Fujita I, et al. Identification of amine acceptor protein substrates of transglutaminase in liver extracts: use of 5-(biotinamido) pentylamine as a probe. Archives of biochemistry and biophysics. 1998 Aug 15; 356(2): 280-286.
[2]. Slaughter TF, Achyuthan KE, Lai TS, et al. A microtiter plate transglutaminase assay utilizing 5-(biotinamido) pentylamine as substrate. Analytical biochemistry. 1992 Aug 15; 205(1): 166-171.
[3]. Ichikawa A, Ishizaki J, Morita M, et al. Identification of new amine acceptor protein substrate candidates of transglutaminase in rat liver extract: use of 5-(biotinamido) pentylamine as a probe. Bioscience, biotechnology, and biochemistry. 2008 Apr 23; 72(4): 1056-1062.
5-(Biotinamido)pentylamine是一种带有C5碳链间隔基的生物素衍生物 [1]。5-(Biotinamido)pentylamine主要用作转谷氨酰胺酶（TG）的胺供体底物。在酶催化下，它可以通过γ-酰胺键与蛋白质中的谷氨酰胺残基共价结合，从而将生物素标签引入目标蛋白 [2-3]。