5α-dihydro-11-keto Testosterone-d3 is intended for use as an internal standard for the quantification of 5α-dihydro-11-keto testosterone by GC- or LC-MS. 5α-dihydro-11-keto Testosterone is a metabolite of 11β-hydroxyandrostenedione and 11β-hydroxytestosterone that has been shown to act as a full androgen receptor agonist with equal potency as that of dihydrotestosterone .1,2,3 The androgenic activity of 5α-dihydro-11-keto testosterone has been implicated in contributing to the androgen pool that drives castration-resistant prostate cancer cells.2,3
1.Bloem, L.M., Storbeck, K.-H., Schloms, L., et al.11β-hydroxyandrostenedione returns to the steroid arena: Biosynthesis, metabolism and functionMolecules18(11)13228-13244(2013) 2.Swart, A.C., and Storbeck, K.-H.11β-hydroxyandrostenedione: Downstream metabolism by 11βHSD, 17βHSD and SRD5A produces novel substrates in familiar pathwayMol. Cell. Endocrinol.408114-123(2015) 3.Storbeck, K.-H., Bloem, L.M., Africander, D., et al.11β-Hydroxydihydrotestosterone and 11-ketodihydrotestosterone, novel C19 steroids with androgenic activity: A putative role in castration resistant prostate cancer•Mol. Cell. Endocrinol.377(1-2)135-146(2013)