5(6)-TAMRA is a fluorescent dye molecule widely used as a label for peptides and proteins, with λem=580nm, λex=523nm[1]. By means of the fluorescence quenching system, 5(6)-TAMRA has been applied to the determination of protease and dehydrogenase activities[2]. 5(6)-TAMRA has been widely used as a model compound for the synthesis of related derivatives and the development of new fluorescent dyes[3].
References:
[1] Shiba A, Kinoshita-Kikuta E, Kinoshita E, et al. TAMRA/TAMRA fluorescence quenching systems for the activity assay of alkaline phosphatase[J]. Sensors, 2017, 17(8): 1877.
[2] Christie R J, Tadiello C J, Chamberlain L M, et al. Optical properties and application of a reactive and bioreducible thiol-containing tetramethylrhodamine dimer[J]. Bioconjugate chemistry, 2009, 20(3): 476-480.
[3] Lyttle M H, Carter T G, Dick D J, et al. A tetramethyl rhodamine (Tamra) phosphoramidite facilitates solid-phase-supported synthesis of 5 ‘-Tamra DNA[J]. The Journal of Organic Chemistry, 2000, 65(26): 9033-9038.
5(6)-TAMRA是一种广泛用于肽和蛋白质标记的荧光染料分子，λem为580nm，λex为523nm[1]。基于荧光淬灭系统，5(6)-TAMRA已被应用于蛋白酶和脱氢酶活性的测定[2]。5(6)-TAMRA还常作为模型化合物，用于相关衍生物的合成以及新型荧光染料的开发[3]。