5(6)-FITC is a fluorescent dye derivative with an excitation/emission wavelength of approximately 492–495nm/518–521nm [1]. The terminal isothiocyanate group (–N=C=S) of 5(6)-FITC can covalently bind to nucleophilic groups such as amino groups and sulfhydryl groups in proteins or nucleic acids [2]. 5(6)-FITC is often used to label biological molecules such as antibodies and oligonucleotides, which facilitates fluorescence microscopy observation, flow cytometry, ELISA, and Western blot experiments [3].
References:
[1]. Miller J S, Quarles J M. Flow cytometric identification of microorganisms by dual staining with FITC and PI[J]. Cytometry: The Journal of the International Society for Analytical Cytology, 1990, 11(6): 667-675.
[2]. Twining S S. Fluorescein isothiocyanate-labeled casein assay for proteolytic enzymes[J]. Analytical biochemistry, 1984, 143(1): 30-34.
[3]. Oddone N, Zambrana A I, Tassano M, et al. Cell uptake mechanisms of PAMAM G4-FITC dendrimer in human myometrial cells[J]. Journal of nanoparticle research, 2013, 15(7): 1776.
5(6)-FITC是一种荧光染料衍生物，激发/发射波长约为492–495nm/518–521nm [1]。5(6)-FITC的末端异硫氰酸酯基团（-N=C=S）可以与蛋白质或核酸中的氨基、巯基等亲核基团共价结合 [2]。5(6)-FITC常用于标记抗体、寡核苷酸等生物分子，方便荧光显微镜观察、流式细胞术、ELISA和蛋白质印迹实验 [3]。