5-(4,6-Dichloro-s-triazin-2-ylamino)fluorescein (DTAF) is an amine-reactive fluorescent probe for labeling proteins.1 It displays excitation/emission maxima of 492/513 nm, respectively. DTAF has been conjugated to IgG for use as fluorescent antibodies and as a pre-column or on-column reagent to label amino acids and amino acid-containing compounds.1,2,3 It has also been used as a derivatizing agent in the analysis of ephedrine and pseudoephedrine from various sources.3
1.Bakeslee, D., and Baines, M.G.Immunofluorescence using dichlorotriazinylaminofluorescein (DTAF). I. Preparation and fractionation of labelled IgGJ. Immunol. Methods13(3-4)305-320(1976) 2.De Boer, S.H., and Wieczorek, A.Production of monoclonal antibodies to Corynebacterium spedonicumPhytopathology74(12)1431-1434(1984) 3.Wang, W., Li, C., Li, Y., et al.Rapid and ultrasensitive determination of ephedrine and pseudoephedrine derivatizated with 5-(4,6-dichloro-s-triazin-2-ylamino) fluorescein by micellar electrokinetic chromatography with laser-induced fluorescence detectionJ. Chromatogr. A.1102(1-2)273-279(2005)