4-Nitrophenyl β-D-Cellobioside is a synthetic chromogenic substrate used for the determination of β-glucosidase activity. 4-Nitrophenyl β-D-Cellobioside is widely used in biochemical research to study the enzymatic hydrolysis of glycosidic bonds[1]. When 4-Nitrophenyl β-D-Cellobioside is hydrolyzed by β-glucosidase, p-nitrophenol is released, which can be easily detected by spectrophotometry at a wavelength of 405nm. 4-Nitrophenyl β-D-Cellobioside is commonly used in the study of carbohydrate metabolism, enzyme kinetics, and the evaluation of enzyme inhibitors[2].
References:
[1] Haataja T, Gado JE, Nutt A, et al. Enzyme kinetics by GH7 cellobiohydrolases on chromogenic substrates is dictated by non-productive binding: insights from crystal structures and MD simulation. FEBS J. 2023 Jan;290(2):379-399.
[2] Mizuma T, Fuseda N, Hayashi M. Kinetic characterization of glycosidase activity from disaccharide conjugate to monosaccharide conjugate in Caco-2 cells. J Pharm Pharmacol. 2005 May;57(5):661-4.
4-Nitrophenyl β-D-Cellobioside是定β - 葡糖苷酶活性的人工合成的显色底物，在生化研究中广泛用于研究糖苷键的酶促水解[1]。当4-Nitrophenyl β-D-Cellobioside被β - 葡糖苷酶水解时，会释放出对硝基苯酚，可容易通过在405nm波长处的光谱光度法检测，常用于研究碳水化合物代谢、酶动力学以及酶抑制剂的评估[2]。