4-Methylumbelliferyl-β-D-Galactoside is a fluorescent substrate that is used for the enzyme release assay to detect human NK cell activity[1]. 4-Methylumbelliferyl-β-D-Galactoside is hydrolyzed during the enzymatic reaction, and the released 4-methylumbelliferone could be quantified by fluorometry [2]. 4-Methylumbelliferyl-β-D-Galactoside can act as the substrate for rapid detection of total and fecal coliform bacteria (TC and FC) in drinking water [3]. 4-Methylumbelliferyl-β-D-Galactoside is currently used to develop a series of highly sensitive assays to probe β-D-galactoside activity in cells[4].
References:
[1] Ohmori H, Ikeda H, Tanigawa T, et al. Enzyme release assay of human NK cell activity using β-galactosidase-expressing K562 target cell line[J]. Journal of immunological methods, 1993, 164(1): 131-135.
[2] Hattenberger M, Mascher F, Kalcher K, et al. Improved method for the fluorimetric detection of β-D-galactosidase in water[J]. International journal of hygiene and environmental health, 2001, 203(3): 281-287.
[3] Berg J D, Fiksdal L. Rapid detection of total and fecal coliforms in water by enzymatic hydrolysis of 4-methylumbelliferone-beta-D-galactoside[J]. Applied and environmental microbiology, 1988, 54(8): 2118-2122.
[4] Ramsay J P. High-throughput β-galactosidase and β-glucuronidase assays using fluorogenic substrates[J]. Bio-protocol, 2013, 3(14): e827-e827.
4-Methylumbelliferyl-β-D-Galactoside是一种荧光底物，可用于酶释放法检测人类自然杀伤（NK）细胞活性[1]。4-Methylumbelliferyl-β-D-Galactoside在酶促反应中被水解，释放出的4-甲基伞形酮可通过荧光测定法进行定量[2]。4-Methylumbelliferyl-β-D-Galactoside可作为快速检测饮用水中总大肠菌群和粪大肠菌群（TC/FC）的底物[3]。4-Methylumbelliferyl-β-D-Galactoside目前被用于开发一系列高灵敏度检测方法以探测细胞内的β-D-半乳糖苷酶活性[4]。