4-hydroxy Nonenal-d3 (4-HNF-d3) contains three deuterium atoms at the terminal methyl position. It is intended for use as an internal standard for the quantification of 4-HNE by GC- or LC-mass spectrometry. 4-HNE is a lipid peroxidation product derived from oxidized ω-6 polyunsaturated fatty acids such as arachidonic acid.1,2 4-HNE is widely used as a marker of lipid peroxidation.2 It exhibits various biological activities such as cytotoxicity, growth inhibiting activity, genotoxicity, and chemotactic activity.1,2,3 4-HNE inhibits pro-oxidant-induced Ca2+ release from mitochondria at 10-50 µM.2
References:
1.Pryor, W.A., and Porter, N.A.Suggested mechanisms for the production of 4-hydroxy-2-nonenal from the autoxidation of polyunsaturated fatty acidsFree Radical Biology & Medicine8541-543(1990).
2.Esterbauer, H., Schaur, R.J., and Zoliner, H.Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde, and related aldehydesFree Radical Biology & Medicine1181-128(1991).
3.Sodum, R.S., and Chung, F.L.1,N2-ethenodeoxyguanosine as a potential marker for DNA adduct formation by trans-4-hydroxy-2-nonenalCancer Research48320-323(1988).
