4-Azido-L-phenylalanine (p-Azidophenylalanine) is an unnatural amino acid analog of L-phenylalanine that contains an azide moiety. 4-Azido-L-phenylalanine (p-Azidophenylalanine)-labeling is a fast, sensitive, and non-radioactive alternative to the traditional technique for detecting nascent protein synthesis.
4-Azido-L-phenylalanine (p-Azidophenylalanine) is randomly incorporated into synthesizing protein instead of phenylalanine during translation. The resulting azide-labeled full-length proteins can be detected via copper-catalyzed click reaction (with fluorescent or biotin-labeled alkynes) or copper-free click reaction (with cycloalkynes) and used for subsequent microscopic imaging or purification tasks.
References:
[1].Peng Y F. Methodology to Site-Specifically Label SBDS Domains to Monitor Dynamics of 60S Ribosomal Subunit Interactions[D]. Saint Louis University, 2023.
4-Azido-L-phenylalanine (p-Azidophenylalanine)是L-苯丙氨酸的非天然氨基酸类似物，含有叠氮部分。4-Azido-L-phenylalanine (p-Azidophenylalanine)标记是一种快速、灵敏且无放射性的替代传统检测新生蛋白质合成技术的方法。
在翻译过程中，4-Azido-L-phenylalanine (p-Azidophenylalanine)代替苯丙氨酸随机掺入合成蛋白质中。所得的叠氮化物标记的全长蛋白质可以通过铜催化的点击反应（使用荧光或生物素标记的炔烃）或无铜点击反应（使用环炔烃）进行检测，并用于后续的显微成像或纯化任务。