3X HA Tag is a bioactive peptide composed of the 9-amino-acid sequence YPYDVPDYA repeated 3 times[1]. HA Tag is derived from the hemagglutinin (HA) surface glycoprotein of human influenza virus, featuring high specificity and hydrophilicity. It enhances the possibility of antigen - antibody reactions on the surface of fusion proteins[2]. With more repeats, 3X HA Tag has stronger antigenicity and higher binding affinity for anti - HA antibodies, ensuring higher detection sensitivity, especially for low - abundance proteins[3]. It's widely used in protein affinity purification experients to elute HA-tagged proteins[4][5].
References:
[1] Schneider, B. L., Seufert, W., Steiner, B., Yang, Q. H., & Futcher, A. B. (1995). Use of polymerase chain reaction epitope tagging for protein tagging in Saccharomyces cerevisiae. Yeast (Chichester, England), 11(13), 1265–1274.
[2] Zhao, X., Li, G., & Liang, S. (2013). Several affinity tags commonly used in chromatographic purification. Journal of analytical methods in chemistry, 2013, 581093.
[3] Ensinck, M., De Keersmaecker, L., Heylen, L., Ramalho, A. S., Gijsbers, R., Farré, R., De Boeck, K., Christ, F., Debyser, Z., & Carlon, M. S. (2020). Phenotyping of Rare CFTR Mutations Reveals Distinct Trafficking and Functional Defects. Cells, 9(3), 754.
[4] Lim, J., Iftner, T., & Simon, C. (2021). Native Isolation of 3×HA-Tagged Protein Complexes to Characterize Protein-Protein Interactions. Current protocols, 1(2), e29.
[5] Tanaka, T., Umemori, T., Endo, S., Muramatsu, S., Kanemaki, M., Kamimura, Y., Obuse, C., & Araki, H. (2011). Sld7, an Sld3-associated protein required for efficient chromosomal DNA replication in budding yeast. The EMBO journal, 30(10), 2019–2030.
3X HA Tag是一种生物活性肽，由9个氨基酸残基（YPYDVPDYA）重复3次构成[1]。HA Tag来源于人类流感病毒的血凝素（HA）表面糖蛋白，具有高度的特异性和亲水性，能够增加融合蛋白表面抗原抗体反应的可能性[2]。3X HA Tag由于重复次数更多，抗原性更强，与抗HA抗体的结合亲和力更高，因此在检测时灵敏度更高，尤其适用于低表达蛋白的检测[3]。它广泛应用于蛋白广泛应用于蛋白亲和纯化实验中以洗脱HA标记的蛋白[4][5]。