306-O12B is an ionizable cationic lipid used to generate lipid nanoparticles (LNPs). It can be used for liver-specific CRISPR-Cas9 gene editing[1].
306-O12B LNP specifically delivered Cas9 mRNA and sgAngptl3 to liver hepatocytes of wild-type C57BL/6 mice, resulting in a median editing rate of 38.5% and a corresponding 65.2% reduction of serum ANGPTL3 protein. Delivery using 306-O12B LNP was more efficient than delivery with MC-3 LNP, a gold standard LNP[1].
Reference：
[1]: Qiu M, Glass Z,et,al. Lipid nanoparticle-mediated codelivery of Cas9 mRNA and single-guide RNA achieves liver-specific in vivo genome editing of Angptl3. Proc Natl Acad Sci U S A. 2021 Mar 9;118(10):e2020401118. doi: 10.1073/pnas.2020401118. PMID: 33649229; PMCID: PMC7958351.
306-O12B是一种可电离的阳离子脂质，用于生成脂质纳米颗粒(LNPs)。可用于肝脏特异性CRISPR-Cas9基因编辑[1]。
306-O12B LNP特异性地将Cas9 mRNA和sgAngptl3传递到野生型C57BL/6小鼠的肝细胞，导致中位编辑率为38.5%，相应的血清ANGPTL3蛋白减少65.2%。306-O12B LNP比MC-3 LNP(金标准)更有效 [1]。