28-Homobrassinolide is a phytosteroid. 28-Homobrassinolide can be used for the research of cholesterol and glucose homeostasis[1].
28-Homobrassinolide (5-20 μM) 表明 HepG2 细胞中的葡萄糖利用以及 ABCA1 和 SREBP2 蛋白表达增加了 2 倍[1]。
28-Homobrassinolide 降低组织葡萄糖和胆固醇水平，增加胆固醇水平和组织己糖激酶活性[1]。
Cell Cytotoxicity Assay[1]
| Cell Line: | HepG2 cells |
| Concentration: | 1 µM, 5 µM, 10 µM, 20 µM, 30 µM, 40 µM, and 80 µM |
| Incubation Time: | 24 h |
| Result: | The IC50 value of 40.57µM obtained by the MTT assay. |
Western Blot Analysis[1]
| Cell Line: | HepG2 cells |
| Concentration: | 5, 10, and 20 μM |
| Incubation Time: | 12 h |
| Result: | Exhibited an increase in both ABCA1 and SREBP2 markers. |
28-HB（1-20 μg/天；持续 6 周）显示高脂肪饮食喂养的 C57BL/6 小鼠的主动脉脂肪沉积和血清标志物水平显着降低[1]。
| Animal Model: | High-fat diet-fed C57BL/6 mice[1] |
| Dosage: | 1-20 μg/day |
| Administration: | For 6 weeks |
| Result: | Observed a significant decrease in lipid deposition. |
[1]. Victor Mukherjee, et al. Phytosteroid 28-homobrassinolide targets cholesterol and glucose homeostasis implicating ABCA1 and SREBP role in regulation. Steroids. 2021 Jan;165:108756.