2’-Deoxyuridine-d2 is intended for use as an internal standard for the quantification of 2’-deoxyuridine by GC- or LC-MS. 2’-Deoxyuridine is a pyrimidine nucleoside composed of the pyrimidine base uracil attached to the sugar deoxyribose.1 It has been used as a precursor in the synthesis of antiviral deoxyuridine derivatives, including idoxuridine .2,3 Formulations containing 2’-deoxyuridine have been used in the deoxyuridine suppression test to identify vitamin B12 and/or folate deficiency and in the diagnosis of megaloblastic anemias.
1.MartÍ, R., LÓpez, L.C., and Hirano, M.Assessment of thymidine phosphorylase function: Measurement of plasma thymidine (and deoxyuridine) and thymidine phosphorylase activityMethods Mol. Biol.837121-133(2012) 2.Prusoff, W.H.Synthesis and biological activities of iododeoxyuridine, an analog of thymidineBiochim. Biophys. Acta.32(1)295-296(1959) 3.Bergstrom, D.E., and Ogawa, M.K.C-5 substituted pyrimidine nucleosides. 2. Synthesis via olefin coupling to organopalladium intermediates derived from uridine to 2’-deoxyuridineJ. Am. Chem. Soc.100(26)8106-8112(1978)