2',3'-Dideoxyadenosine-5'-O-triphosphate (sodium salt)

2',3'-Dideoxyadenosine-5'-O-triphosphate is a reverse transcriptase inhibitor (K_is = 20 and 37 nM for the HIV and visna virus enzymes, respectively) and an active metabolite of 2′,3′-dideoxyadenosine and didanosine .^1,2,3 It is formed from ddI via a 2',3'-dideoxyAMP intermediate by phosphoribosyl pyrophosphate synthase (PRS).³ It inhibits DNA polymerase isolated from mouse Ehrlich ascites tumor cells in the presence of ATP or GDP (K_is = 60 and 18 µM, respectively).⁴ 2',3'-Dideoxyadenosine-5'-O-triphosphate and other dideoxynucleoside 5'-triphosphates are commonly used to terminate chain extension by Taq polymerases in PCR assays.⁵