13,14-dihydro Prostaglandin E1-d4

13,14-dihydro Prostaglandin E₁-d₄ (13,14-dh PGE₁-d₄) contains four deuterium atoms at the 3, 3', 4, and 4' positions. It is intended for use as an internal standard for the quantification of 13,14-dh PGE₁ by GC- or LC-mass spectrometry. 13,14-dh PGE₁ is an inhibitor of ADP-induced platelet aggregation in human platelet-rich plasma and washed platelets with IC₅₀ values of 31 and 21 nM, respectively.¹ 13,14-dh PGE₁ is a slightly more potent inhibitor of ADP-induced human platelet aggregation than PGE₁ which has an IC₅₀ of 40 nM.² Also, 13,14-dh PGE₁ activates adenylate cyclase in NCB-20 hybrid cells with a Kact of 668 nM.³

1.Kobzar, G., Mardla, V., JÄrving, I., et al.Comparison of the inhibitory effect of E-prostaglandins in human and rabbit platelet-rich plasma and washed plateletsComparative Biochemistry and Physiology106(2)489-494(1993) 2.Kobzar, G., Mardla, V., JÄrving, I., et al.Antiaggregating potency of E-type prostaglandins in human and rabbit plateletsProceedings of the Estonian Academy of Sciences.Chemistry40179-180(1991) 3.Blair, I.A., Hensby, C.N., and MacDermot, J.Prostacyclin-dependent activation of adenylate cyclase in a neuronal somatic cell hybrid: Prostanoid structure-activity relationshipsBr. J. Pharmac.69(3)519-525(1980)