1,2-Dioleoyl-sn-glycero-3-PC (DOPC) is an unsaturated phospholipid containing two oleic acid chains (18:1) and is commonly used for the formation of micelles, liposomes, and other types of artificial membranes[1]. 1,2-Dioleoyl-sn-glycero-3-PC can incorporate purified proteins from dodecyl-β-D-maltopyranoside (DDM) to form mixed PC-DDM micelles [2]. It can form stable phospholipid bilayers, which are utilized to study structural changes in multi-component biological membranes under various physiological conditions [3]. 1,2-Dioleoyl-sn-glycero-3-PC is also used for the encapsulation of drugs, proteins, and nucleic acids [4]. As a neutral lipid, 1,2-Dioleoyl-sn-glycero-3-PC has minimal impact on cell signaling and can be used as a control to distinguish the specific effects of phosphatidic acid (PA) on Hippo signaling [5].
References:
[1] Efrat M, Rosenblat M, Mahmood S, et al. Di-oleoyl phosphatidylcholine (PC-18: 1) stimulates paraoxonase 1 (PON1) enzymatic and biological activities: in vitro and in vivo studies[J]. Atherosclerosis, 2009, 202(2): 461-469.
[2] Caldo K M P, Shen W, Xu Y, et al. Diacylglycerol acyltransferase 1 is activated by phosphatidate and inhibited by SnRK1‐catalyzed phosphorylation[J]. The Plant Journal, 2018, 96(2): 287-299.
[3] Leonenko Z V, Finot E, Ma H, et al. Investigation of temperature-induced phase transitions in DOPC and DPPC phospholipid bilayers using temperature-controlled scanning force microscopy[J]. Biophysical journal, 2004, 86(6): 3783-3793.
[4] Kandregula B, Narisepalli S, Chitkara D, et al. Exploration of lipid-based nanocarriers as drug delivery systems in diabetic foot ulcer[J]. Molecular Pharmaceutics, 2022, 19(7): 1977-1998.
[5] Tei R, Baskin J M. Spatiotemporal control of phosphatidic acid signaling with optogenetic, engineered phospholipase Ds[J]. Journal of Cell Biology, 2020, 219(3): e201907013.
1,2-Dioleoyl-sn-glycero-3-PC（DOPC）是一种不饱和磷脂，含有两条油酸链 （18:1），通常用于胶束、脂质体和其他类型的人造膜的生成[1]。1,2-Dioleoyl-sn-glycero-3-PC可将正十二烷基-β-d-吡喃麦芽糖苷（DDM）中的纯化蛋白质脂化，形成混合PC-DDM胶束[2]。1,2-Dioleoyl-sn-glycero-3-PC可形成稳定的磷脂双层，用于研究不同生理条件下多组分生物膜的结构变化[3]。1,2-Dioleoyl-sn-glycero-3-PC可用于封装药物、蛋白质和核酸[4]。1,2-Dioleoyl-sn-glycero-3-PC是一种中性脂质，对细胞信号传导的影响很小，可以作为对照，用来区分磷脂酸（PA）对 Hippo 信号传导的具体影响[5]。