1,2-Dioleoyl-rac-glycerol是一种重要的二酰基甘油酯，在质谱分析中用作脂质标准品。
Cas No.:2442-61-7
Sample solution is provided at 25 µL, 10mM.
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1,2-Dioleoyl-rac-glycerol, an important diacylglyceride, is used as the lipid standard in mass spectrometry analysis [1]. 1,2-Dioleoyl-rac-glycerol inhibits phorbol ester binding to protein kinase C via a competitive mechanism [2]. 1,2-Dioleoyl-rac-glycerol is widely used as an helper lipid in gene delivery systems to enhance gene transfer[3].
In vitro, 1,2-Dioleoyl-rac-glycerol treatment at 50μM for 5 minutes significantly induced the expression of protein kinase C in myoblasts and increased calcium ion influx[4].
In vivo, 1,2-Dioleoyl-rac-glycerol treatment via subcutaneous injection at a dose of 150μg/100g/day for 6 days inhibited the tumor growth in the L5178Y xenograft tumor mouse models[5].
References:
[1] Sämfors S, Ewing A G, Fletcher J S. Benefits of NaCl addition for ToF-SIMS analysis including the discrimination of diacylglyceride and triacylglyceride ions[J]. Rapid communications in mass spectrometry: RCM, 2018, 32(17): 1473.
[2] Sharkey N A, Blumberg P M. Kinetic evidence that 1, 2-diolein inhibits phorbol ester binding to protein kinase C via a competitive mechanism[J]. Biochemical and biophysical research communications, 1985, 133(3): 1051-1056.
[3] Kichler A, Mechtler K, Behr J P, et al. Influence of membrane-active peptides on lipospermine/DNA complex mediated gene transfer[J]. Bioconjugate chemistry, 1997, 8(2): 213-221.
[4] Capiati D A, Vazquez G, Tellez Iñón M T, et al. Role of protein kinase C in 1, 25 (OH) 2‐vitamin D3 modulation of intracellular calcium during development of skeletal muscle cells in culture[J]. Journal of cellular biochemistry, 2000, 77(2): 200-212.
[5] Fukusen N, Kido H, Kato Y, et al. Diacylglycerols Enhance the Anti‐tumor Effect of Glucocorticoid on L5178Y Lymphoblasts in vivo[J]. Japanese journal of cancer research, 1989, 80(10): 963-967.
1,2-Dioleoyl-rac-glycerol是一种重要的二酰基甘油酯，在质谱分析中用作脂质标准品[1]。1,2-Dioleoyl-rac-glycerol通过竞争性机制抑制佛波酯与蛋白激酶C的结合[2]。1,2-Dioleoyl-rac-glycerol作为辅助脂质被广泛用于基因递送系统，以增强基因转移效率[3]。
在体外，使用50μM的1,2-Dioleoyl-rac-glycerol处理成肌细胞5分钟，显著诱导了蛋白激酶C的表达并增加了钙离子内流[4]。
在体内，通过皮下注射给予150μg/100g/day剂量的1,2-Dioleoyl-rac-glycerol，连续6天，抑制了L5178Y异种移植肿瘤小鼠模型中的肿瘤生长[5]。
| Animal experiment [1]: | |
Animal models | Male DBA/2 mice |
Preparation Method | L5178Y lymphoblasts from in vitro cultures at 37°C in Ham's F12 medium with 10% fetal calf serum were inoculated s.c. (5×106 cells in 0.1ml of saline) into the first inguinal gland of groups of 10 male DBA/2 mice, weighing 20 to 22g, on experimental day 1. The mice were given laboratory chow and water ad libitum. TPA or 1,2-Dioleoyl-rac-glycerol (150μg/100g) in saline containing 10% dimethyl sulfoxide and/or triamcinolone acetonide in saline containing 10% ethanol in a total volume of 0.2ml was simultaneously injected s.c. into the rump of the mice once a day for 6 days from day 2. Mice were sacrificed on day 8, and the excised tumor without the capsule was weighed. |
Dosage form | 150μg/100g/day for 6 days; s.c. |
Applications | 1,2-Dioleoyl-rac-glycerol treatment inhibited the tumor growth in the L5178Y xenograft tumor mouse models. |
References: | |
| Cas No. | 2442-61-7 | SDF | |
| 别名 | 甘油1,2-二油酸酯,1,2-Dioleoyl-rac-glycerol | ||
| Canonical SMILES | O=C(OCC(CO)OC(CCCCCCC/C=C\CCCCCCCC)=O)CCCCCCC/C=C\CCCCCCCC | ||
| 分子式 | C39H72O5 | 分子量 | 621 |
| 溶解度 | DMF: 10 mg/ml,Ethanol: 10 mg/ml,Ethanol:PBS(pH 7.2) (1:1): .5 mg/ml | 储存条件 | Store at RT |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.6103 mL | 8.0515 mL | 16.1031 mL |
| 5 mM | 322.1 μL | 1.6103 mL | 3.2206 mL |
| 10 mM | 161 μL | 805.2 μL | 1.6103 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >99.00% Appearance: A solution in acetonitrile
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