1,2-Dioleoyl-3(R)-trimethylammoniumpropane (chloride)

1,2-Dioleoyl-3(R)-trimetlammoniumpropane (R-DOTAP) is a cationic lipid and the more immunologically active isomer of DOTAP .^1,2 Lipoplexes containing R-DOTAP and encapsulating siRNA targeting the gene encoding aromatase, CYP19A1, decrease aromatase activity in MCF-7 breast cancer cells.¹ Vaccination with a peptide epitope from human papillomavirus (HPV) 16 protein E7 in R-DOTAP-containing liposomes induces IFN-γ production by CD8⁺ T cells and tumor-infiltrating lymphocytes (TILs) and decreases tumor size in a murine cervical cancer model.² Subcutaneous immunization with recombinant influenza B hemagglutinin using R-DOTAP as an adjuvant increases IL-2 and IFN-γ production in CD4⁺ T cells isolated from mouse draining lymph nodes and spleen.³